Normalization software pressure

The energy involved in the folding and association of copolymer chains in solutions can be measured by a micro-calorimeter (MicroCal Inc). We used US-DSC at an external pressure of 180 kPa. The cell volume is only 0.157 mL. The heating rate can be varied and the instrument response time is normally a few seconds. All the DSC data should be corrected for instrument response time and can be analyzed using the software in the calorimeter. Note that the concentration used in DSC is normally not lower than 10-3 g/mL, much higher than that used in LLS (10 6-10 3 g/mL). 

Figure 3 shows a half-normal probability plot of the effect estimates obtained from the Design-Expert software package. Three main effects, A (pressure), B (power), and E (gap) are important. Because the main effects are aliased with three-factor interactions, this interpretation is probably correct. There are also two two-factor interaction alias chains that are important, AB = CE and AC = BE. Because AB is the interaction of two strong main effects, those of pressure and... 

Statistical analysis was formed by using a commercially available computer programme (Analyse-It, Andyse-It Software Ltd, Leeds, UK). Differences between various pressures and LD blood flow values over one cycle were analysed using Student s t-test or the Wilcoxon s signed rank test depending on whether or not data were normally distributed. A difference was considered significant when p<0.05. 

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