Nordeoxycholic acid

Sada, K. Sugahara, M. Nakahata. Y. Yasuda. Y. Nishio. A. Miyata, M. First columnar monolayer stracture of bile acids inclusion crystal. Inclusion compounds of 23-nordeoxycholic acid. Chem. Lett. 1998. 31. 

In the procedure of Evrard and Janssen (23), freeze-dried human feces is extracted with hot acetic acid containing nordeoxycholic acid (internal standard). Toluene is added and the supernatant removed and taken to dryness. The residue is dissolved in alkaline ethylene glycol and refluxed for 20 min at 220 X. Unsaponifiable compounds are next removed by petroleum ether extraction, the acidic compounds in the remaining aqueous phase are extracted with diethyl ether after acidification. The free bile acids thus obtained are methylated, oxidized with Cr03 in 90% aqueous acetic acid, and recovered by diethyl ether extraction. After evaporation of the ether the ketonic bile acid methyl esters are determined by temperature-programmed gas-liquid chromatography on a 1 % JXR-column. 

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