Noradrenaline release from synaptosomes

Dekker LV, De Graan PN, Pijnappel P et al (1991) Noradrenaline release from streptolysin O-permeated rat cortical synaptosomes effects of calcium, phorbol esters, protein kinase inhibitors, and antibodies to the neuron-specific protein kinase C substrate B-50 (GAP-43). J Neurochem 56 1146-53... 

In ex vivo studies in which transmitter release is measured in synaptosome or slice preparations from animals treated with nicotine in vivo, no change (Grilli et al. 2005), decreased (Grady et al. 1997) and increased (Yu and Wecker 1994) striatal [ HJdopamine release has been reported. A similar picture has been observed for nAChR-evoked hippocampal [ H]noradrenaline release, with unchanged (Barik and Wonnacott 2006), decreased (Grilli et al. 2005) and increased (Jacobs et al. 2002) responses. As tissue is extensively washed prior to nAChR stimulation, these discrepancies are unlikely to reflect differences in nAChR desensitisation due... 

Clarke PB, Reuben M. (1996). Release of [3FI]-noradrenaline from rat hippocampal synaptosomes by nicotine mediation by different nicotinic receptor subtypes from striatal [3FI]-dopamine release. BrJ Pharmacol. 117(4) 595-606. 

Fig. 3. Ca dependent (vesicular) release of multiple neurotransmitter types from rat hippocampal synaptosomes. To evoke release, the extracellular K concentration was raised to 30 mA/by adding KCl. The basis of the neurotransmitter type categories (Type I, and so forth) is described in Note 19. The measurements are expressed in log[fmol/mg protein]. See Section 3.4, and Note 21 for further discussion. GLU = glutamate, NA = noradrenaline, DA = dopamine, CCK = cholecystokinin, met-Enk = met-enkephalin.

Bennett, G. W., Marsden, C. A., Sharp, T., and Stolz, J. F., 1981a, Concomitant determination of endogenous release of dopamine, noradrenaline, 5-hydroxytryptamine, and thyrotrophin-releasing hormone (TRH) from rat brain slices and synaptosomes, in Central Neurotransmitter Turnover (C. J. Pycock and P. V. Taberner, eds.), pp. 183-189, Croom-Helm, London. 

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