Nonviral siRNA delivery carriers

The accessibility of different tissue types, the presence of various delivery routes, and a variety of pharmacological requirements makes it impossible to have a universal in vivo delivery system suitable to every scenario of siRNA delivery, hi terms of in vivo delivery vehicles for siRNA, the nonviral carriers are the major type being investigated so far, though some physical and viral delivery approaches are also very effective. The routes of in vivo deliveries are commonly categorized as local or systemic. Some of the delivery vehicles and dehvery routes are very effective in animals for target validation but may not be useful for delivery of siRNA therapeutics in humans (Fig. 3.3). Therefore, in vivo siRNA delivery carriers and methods can also be classified as clinically viable and nonclinically viable, according to their suitability for the human use. 

Nonviral carriers have been examined in attempts to protect and try to achieve intracellular delivery of oligonucleotides such as plasmid DNA and siRNA [109]. Polyacetals would be expected to undergo enhanced rates of degradation upon endocytic uptake. In addition to the aforementioned acetal/ketal polyamidoamines 23 (Figure 13.5b),... 

Keywords Biocompatibility Biosignal sensitivity Gene delivery Nonviral carriers Oligodeoxynucleotide (ODN) delivery Polyion complex (PIC) micelle Polymeric carriers siRNA dehvery... 

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