Transposition nonreplicative

Nonreplicative transposition. The transposable element is spliced out of its original site (donor site) and inserted into the target site. Then the donor site must be repaired. If it cannot be repaired by the cell s DNA repair system, the consequences will be lethal. 

Whether transposition is replicative or nonreplicative, short duplications of target site DNA segments are generated by the staggered cleavage catalyzed by trans-posase (Figure 18.17). 

Eukaryotic transposons move in a nonreplicative manner via excision and integration (cut and paste). For transposition, the transposase binds to the IRs and cleaves the DNA, thereby precisely excising the transposon. Additionally, the transposase binds to the target DNA, introducing a staggered cut, leading to... 

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