Nondisruptive deletions

The ideal mutation is a nondisruptive deletion that is, one that simply removes an interaction without causing a disruption or reorganization of structure. A formal analysis shows that measurements on nondisruptive deletants provide information on the energetic changes in the wild-type enzyme.11... 

A difference energy diagram constructed for nondisruptive deletion mutations in wild-type enzyme shows at a glance the role of the target side chains in the wild type. This is seen most clearly in the difference energy diagrams plotted in Figure 15.4 for the mutation of Thr-40 to Ala or Gly and for the mutation of His-45... 

Nitrocellulose filters 205 Non-Arrhenius kinetics 555, 598 Nonbonded interactions 325-332 Nondisruptive deletion 426, 560 Nonequilibrium dialysis 202 Nonproductive binding 114-118, 371-372... 

The ideal mutation for enzyme studies is a nondisruptive deletion, i.e. only ranov-ing an interaction without causing a dismption or reorganization of protein structure. The alternations (mutations) causing least structural effect are ... 

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