Noncovalent labeling

Sloat AL, Roper MG, Lin X, Ferrance JP, Landers JP, Colyer CL (2008) Protein determination by microchip capillary electrophoresis using an asymmetric squarylium dye noncovalent labeling and nonequilibrium measurement of association constants. Electrophoresis 29 3446-3455... 

Patonay G, Salon J, Sowell J, Strekowski L (2004) Noncovalent labeling of biomolecules with red and near-infrared dyes. Molecules 9 40-49... 

ED Moody, PJ Viskari, CL Colyer. Noncovalent labeling of human serum albumin with indocyanine green a study by capillary electrophoresis with diode laser-induced fluorescence detection. J Chromatogr B 729 55-64, 1999. 

Fenn JB, Mann M, Meng CK Electrospray ionization for mass spectrometry of large biomolecules. Science (1989) 246 64-71. Patrick JS, Lagu AL Review applications of capillary electrophoresis to the analysis of biotechnology-derived therapeutic proteins. Electrophoresis (2001) 22 4179-4196. Sowell J, Salon J, Strekowski L, et al Covalent and noncovalent labeling schemes for near-infrared dyes in capillary electrophoresis protein applications. Methods Mol. Biol. (2004) 276 39-75. Moini M Capillary electrophoresis mass spectrometry and its application to the analysis ofbiological mixtures. Anal. Bio-anal. Chem. (2002) 373 466 180. Nemunaitis J, Holmlund JT, Kraynak M, et al. Phase I evaluation of ISIS 3521, an antisense oligodeoxynucleotide to protein kinase C-a, in patients with advanced cancer./. Clin. Oncol. (1999) 17 3586-3595. De Frutos M, Cifuentes A, Diez-Masa JC Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin. Electrophoresis (2003) 24 678-680. 

Prakash et al. [248] visualized individual CNTs by fluorescence microscopy through noncovalent labeling with conventional fluorophores (Fig. 1.19). Reversal of contrast in fluorescence imaging of the CNTs was observed when the labeling procedure was performed in a nonpolar solvent. The results were consistent with a CNT-fluorophore affinity mediated by hydrophobic interaction, the reverse-contrast images also provided a clear indication of the nanotube location [248]. 

Fig. 1.19 Chemical structures of conventional fluorophores used for noncovalent labeling of individual CNTs for fluorescence microscopy.

Improving specificity of Binary aptamer Noncovalent labeling Fluorescent sensing 37... 

Sowell J, Salon J, Strekowski L, Patonay G Covalent and noncovalent labeling schemes for near-infrared dyes in capillary electrophoresis protein applications. Methods Mol Biol 2004 276 39-75. Heegaard NH, Nilsson S, Guzman NA. Affinity capillary electrophoresis important application areas and some recent developments. J Chromatogr B Biomed Sci Appl 1998 715 29-54. 

---