Noncoding strand

In the present example we have examined the sequence in mRNA. In the DNA there are two strands. One is the coding strand (also called the nontranscribing or nontranscribed strand), which has a sequence that corresponds to that in the mRNA and the one that is given in Fig. 5-4. The second antiparallel and complementary strand can be called the template strand or the noncoding, transcribing, or transcribed strand.372 The mRNA that is formed is sometimes referred to as a sense strand. The complementary mRNA, which corresponds in sequence to the noncoding strand of DNA, is usually called antisense RNA. 

Promoters/TFBS databases must be formatted by the user. Each line should include only the start coordinate of the promoter or TFBS (see Note 7) and the strand orientation of the promoter or TFBS (0 for coding strand, 1 for noncoding strand). 

Template strand The strand of DNA that is being copied during nucleic-acid synthesis. The template strand in RNA synthesis represents the noncoding strand and is complementary in sequence to the RNA. 

The strand that the RNA polymerase uses as a template for its RNA is called the template strand, the noncoding strand, the antisense strand, and the (-) strand. The other strand, whose sequence matches the RNA produced except for the T-U change, is called the nontemplate strand, the coding strand, the sense strand, and the (+) strand. 

Figure 1 MW of 46 bp PCR product of mutant human K-ras codon 12 variantTGT determined using ESI-quadrupole MS after clean-up of PCR product by (A) ethanol precipitation or (B) Zip-Tip. The spectra have been deconvoluted to simplify visual discrimination. Predicted masses for the coding and noncoding strands are 14261.3 and 14039.1 Da, respectively.

Figure 2 Measured MW of PCR products, 16S-23S interspace region determined using ESI-quadrupole MS. (A) B. anthracis (89 bp, predicted mass of coding and noncoding strands 27 237.8 and 27618.9, respectively) and (B) B. subtilis (114 bp, predicted mass of coding and noncoding strands 34 972.8 and 35329.0, respectively).

Fig. 6 In vitro transcription in a HeLa cell nuclear extract and SI nuclease mapping of RNA products. OVEC Spl template lane 1, control lane 2, with 50mM Zny-thionein lane 5, with 50mM thionein) SI probe, P-end-labeled 93 nucleotide probe extending between positions —18 and +75 of the noncoding strand of the J -globin gene. M/fapII-digested pBR 322 marker DNA. (Adapted from Fig. 2 of Zeng et al. 1991a)...

Unlike what happens in DNA replication, where both strands are copied, only one of the two DNA strands is transcribed into mRNA. The DNA strand that contains the gene is often called the sense strand, or coding strand, and the DNA strand that gets transcribed to give RNA is called the antisense strand, or noncoding strand. Because the sense strand and the antisense strand in DNA are complementary, and because the DNA antisense strand and the newly formed RNA strand are also complementary, the RNA molecule produced during transcription is a copy of the DNA sense strand. That is, the complement of the complement is the same as the original. The only difference is that the RNA molecule has a U everywhere the DNA sense strand has a T. 

Antisense strand (Section 24.4) The template, noncoding strand of double-helical DNA that does not contain the gene. 

Noncoding strand (Section 28.4) An alternative name for the antisense strand of DNA. 

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