Non-mitochondrial Origin of Eukaryotic Glycolysis

The aforementioned data raise two questions. May conventional phylogenetic methods still apply to glycolytic enzymes If yes, which bacterial group, if not an a subdivision, was a taxonomic source of eukaryotic glycolysis To address these questions, phylogenetic analysis of several enzymes was carried out in the present work based on the following premises  

Among eukaryotes, only crown groups were involved. As far as possible, Protozoa were omitted for several reasons  

Eukaryotic sequences were suitably aligned, then one of them was used as a query to search for finished and unfinished prokaryotic genomes via a BLAST server. If several homologs were found in a single species, only the most similar one was chosen for alignment. In the basis of the BLAST hit, the annotated complete sequence was retrieved from the finished genome when available. 

Neighbour-joining (NJ) analysis with approximate correction for among-sites rate variation was applied to an alignment, which did not incorporate weakly homologous sequences. Species or even phyla characterized by long branches were additionally excluded. 

Finally, bootstrapped analyses were performed with an alignment refined in the above ways. 

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