Non-competitive assays with complement immobilized on the solid phase

Non-competitive assays with complement immobilized on the solid phase Immobilization of Clq on the solid phase can 

Many other capture molecules could also be used (e.g., receptors, lectins) the principles of such methods do not differ from those discussed here. 

Non-competitive assays with Clq immobilized on the solid phase 

Coat solid phase with C Iq (about 2 t g/ml Chapter 13) and store desiccated at 4 C until used in step 3. 

Prepare serial dilutions of the antigen in the wells of a hard microtitre plate (Dyna-tech 223-24) and add to each well an equal volume of antiserum in the appropriate buffer (Table 14.1). Incubate for 3 h at room temperature or 2 h at 37 C (depending on the reactivity of the antibodies at these temperatures). 

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