Nocardia lactamdurans

JJR Coque, JL de la Fuente, P Liras, J Martin. Overexpression of the Nocardia lactamdurans alpha-aminoadipyl-cysteinyl-valine synthetase in Streptomyces lividans. The purified multienzyme uses cystathionine and 6-oxopiperidine 2-carboxylate as substrates for synthesis of the tripeptide. Eur J Biochem 242 264-270, 1996. 

VK Chary, JL de la Fuente, AL Leitao, P Liras, JF Martin. Overexpression of the lat gene in Nocardia lactamdurans from strong heterologous promoters results in very high levels of lysine-6-aminotransferase and up to two-fold increase in cepha-mycin C production. Appl Microbiol Biotechnol 53 282-288, 2000. 

JJR Coque, JF Martin, P Liras. Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans the organization of the cephamycin gene cluster differs from that in Streptomyces clavuligerus. Mol Gen Genet 236 453-458, 1993. 

The biosynthetic source of the pyridone ring of 87 was investigated [260]. Thymine was found to inhibit uracil catabolism and 87 biosynthesis by Nocardia lactamdurans this inhibition was reversed by uracil catabolites. Both [5,6-3H]-uracil and [4,5-13c]-uracil were incorporated, with both labelled carbons of the latter being incorporated as a unit at C(4) and C(5) of 87. The proposed biosynthetic pathway (Scheme 2) involved catabolism of uracil to p-alanine, which was then incorporated into the pyridone ring of 87 [260]. 

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