Proton-decoupled NMR spectra

Fig. 12. P-NMR spectra (proton-decoupled, 109.3 MHz) of hen egg-white ovalbumin at pH 8.3. Assignments of the resonances (a) phosphoserine-68, 5.0 ppm, and phosphoserine-344, 4.75 ppm (b) same as in (a) but 1 h after the addition of 15 mAf MgCl2 (causes a small upheld shift) and E. coli alkaline phosphatase (c) same as in (b) but 20 h after the addition of the phosphatase. The new resonance appearing at 3 ppm is inorganic phosphate liberated by the phosphatase treatment. From Vogel and Bridger (1982c). Copyright 1982 American Chemical Society.

Fig. 10. Temperature dependence of the 81-MHz P-NMR spectra (proton decoupled) of phosphatidylethanolamine dispersed in buffer. From Cullis and Hope (1980).

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