Nitrogenase purification

As indicated, a number of laboratories got into the process of purifying the components of nitrogenase, so it is difficult to credit any particular laboratory with first success in the endeavor In 1972,Tsoetol. [22] reported purification of dinitrogenase and dinitrogenase reductase to the highest specific activities reported up to that time. Preparations from different organisms require modification of the techniques. Winter and Burris [23] reported a table of activities of components prepared up to 1976. 

Tso, M.-Y., T. Ljones and R.H. Burris. Purification of the nitrogenase protein from Clostridium pasteurianum. Biochim. Biophys. Acta 267,600-604 (1972). 

Chisnell, J.R., Premakumar, R., and Bishop, P.E. 1988. Purification of a second alternative nitrogenase from a nif HDK deletion strain of Azotobacter vinelandii. J. Bacteriol. 170, 27-... 

Hallenbeck, P. C., Meyer, C. M., and Vignais, P. M.1982. Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata purification and molecular properties. J. Bacteriol. 149, 708-717. 

V. Klucas, B. Koch, S. A. Russell, and H. J. Evans, Purification and some properties of the nitrogenase from soybean (Glycine max Merr.) nodules. Plant Physiol. 43 1906 (1968). 

Hallenbeck PC, Kostel PH and Benemann JR (1979) Purification and properties of nitrogenase from the cyanobacterium Anabaena cylindrica, Eur. J. Biochem. 98, 275-284. 

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