Nitrogen uptake kinetics

Fan, C. L., Glibert, P. M., and Burkholder, J. M. (2003b). Characterization of the affinity for nitrogen, uptake kinetics, and environmental relationships for Prorocentrum minimum in natural blooms and laboratory cultures. Harmful Algae 2, 283—299. 

Bradley, P. M., and Morris, J. T. (1990). Influence of oxygen and srdfide concentration on nitrogen uptake kinetics in Spartina alternijlora. Ecology 71(1), 282—287. 

Cochlan W.P. and Bronk D.A. 2001. Nitrogen uptake kinetics in the Ross Sea, Antarctica. Deep-Sea Res. II48 4127-4153. 

Kudela, R. M., and Cochlan, W. P. (2000). Nitrogen and carbon uptake kinetics and the influence of irradiance for a red tide bloom off southern California. Aquat. Microb. Ecol. 21, 31—47. 

Herndon, J., Cochlan, W. P. (2007). Nitrogen utilization by the raphidophyte Heterosigma akashiwo Growth and uptake kinetics in laboratory cultures. Harmful Algae 6, 260—270. 

Caperon, J., and Meyer, J. (1972). Nitrogen-limited growth of marine phytoplankton II. Uptake kinetics and their role in nutrient limited growth of phytoplankton. Deep Sea Res. 19, 619—632. 

Table 23.1 Nitrogen uptake parameters for seagrass species based on Michaelis-Menten uptake kinetics...

Figure 30.3 Difference between nitrogen uptake and growth kinetics from experiments, after Zehr et al. (1988). Short-term uptake kinetics (A) are driven by membrane transport processes, and usually exceed assimilation and incorporation into protein. Short-term uptake declines as feedback from internal pools and regulatory mechanisms are affected by influx of N. (B) Longer term N uptake is driven by protein synthesis rate, which is equivalent to the growth rate. (C) Model of competition between two species showing that competition can be affected by both transport and growth.

The growth of immobilized cells ceased once the nitrogen source was exhausted, as reported by Nava et al. (17) (Fig. 3). After that, immobilized cells were released from the immobilization support and became free cells. Gibberellic acid production (0.160 g/L) was effective once the ammonia was exhausted, and it continued until total glucose consumption. In the case of immobilized cells cultured in a stirred reactor, Fig. 4 shows the fermentation kinetics. One can observe some differences related to the immobilized biomass production, and the uptake in nitrogen and carbon sources. However,... 

The Michaelis-Menten equation is often employed in soil-water systems to describe kinetics of ion uptake by plant roots and microbial cells, as well as microbial degradation-transformation of organics (e.g., pesticides, industrial organics, nitrogen, sulfur, and natural organics) and oxidation or reduction of metals or metalloids. Derivation of the Michaelis-Menten equation(s) is demonstrated below. 

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