Nitro triazoles analysis

High-performance liquid chromatography (HPLC) has been used to analyze metronidazole [1435-1437], misonidazole [1309,1438], and other nitroimidazoles [1435, 1439] in body fluids or pharmaceutical dosage forms. HPLC analysis of effect of hypoxic-cell radiosensitizer misonidazole on the radiation-induced reduction of DNA bases (thymine, cytosine, and adenine) has been carried out [1440, 1441], HPLC was employed to characterize different nitroimidazoles [327, 366, 388,409, 450, 1442-1444], nitropyrazoles [246, 301], nitrothiazoles [366], l-aryl(hetaryl)-4-nitro-l,2,3-triazoles [601], nitrobenzimidazoles [707], nitrobenzofurazans [774, 1445-1449], nitrobenzotriazoles [1450],... 

A three-component condensation of 3-amino-l, 2,4-triazole (or its 5-Me and 5-methylthio derivatives), 1 and DMF-dimethylacetal afforded 8,9-dihydro[l,2,4]triazolo[l,5-fl]quinazolin-6(7H)-ones (06RCB1224). Condensation of 3,4,5-triamino-l,2,4-triazole with aromatic aldehydes and dimedone afforded partially hydrogenated 9-aryl-[l,2,4]triazolo[5,l-frJquinazolin-8-ones. The structure of 2-amino-6,6-dimethyl-3-(4-nitro-benzylidene)amino-9-(4-nitrophenyl)-5,6,7,9-tetrahydro[l,2,4]triazolo[5,l-fr]-quinazolin-8-one was confirmed by X-ray analysis (05RCB2903). 

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