NIH-OVCAR

An avidin-biotin system has been used to attach antibodies in the bilayer of DDSs. Xiao et al. developed a three-step strategy to improve the tumor-to-tissue ratio of anticancer agents [184], Two antibodies specific for the CA-125 antigen that is highly expressed on NIH OVCAR-3 cells were used. These cells were prelabeled with biotinylated anti-CA-125 antibody and fluoroscein isothiocyanate (FITC)-labeled streptavidin (SAv) prior to administration of biotinylated liposomes. Both antibodies were specifically bound to the cell surface of OVCAR-3 cells but not to SK-OV-3 cells, which do not express the specific antibody. Antibody biotinylation did not affect its immunoreactivity. 

The human ovarian carcinoma cell line NIH OVCAR-3 originates from ATCC (Manassas, USA). OVCAR-3 cells are passaged when confluency is reached to provide new maintenance cultures in 75 cm culture flasks. For maintenance, in general, 1 10 part of the last passage is transferred into a new flask. This procedure is done twice a week. 

Chao W, Hobbs PD, Jong L, Zhang X, Zheng Y, Wu Q, Shroot B, Dawson MI (1997) Effects of receptor class-and subtype-selective retinoids and an apoptosis-inducing retinoid on the adherent growth of the NIH OVCAR-3 ovarian cancer cell line in culture. Cancer Lett 113 1-7... 

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