Nicotiana tobacum

Glycine max hypocotyls Nicotiana tobacum plants Helianthus annuus plants Sorghum vulgare seedlings Brassica rapa seedlings Vaccinium macrocarpon fruit Euphorbia pulcherrima bracts Amaranthus tricolor seedlings Oryza sativa leaves... 

Source dried leaves of Nicotiana tobacum (2-8%) and rusticum... 

Nicotinoids. Nicotine from tobacco was one of the earliest insecticides and was recommended for use in 1763 as a tea for the destruction of aphids (1,20). Nicotine [54-11 -3], L-l-methyl-2-(3 -pyridyl)pyrrolidine (1) (bp 247°C, d 1.009), is found in the leaves of Nicotiana tobacum and N. rustica (Solanaceae) in amounts ranging from 2 to 14%, and also is found in Duboisia hopwoodii and in Aesclepias syriaca. It occurs as the principal alkaloid along with small amounts of 12 other alkaloids of which nomicotine [494-97-3], 2-(3,-pyridyl)pyrrolidine (2) (bp 270°C, d 1.07 g/mL), and anabasine [494-52-0], l-2-(3 -pyridyl)piperidiae (3) (bp 281°C, d 1.048), are of insecticidal importance (see Alkaloids). Nomicotine occurs as both the D and L forms, the former in D. hopn oodii and the latter commonly predominating in Nicotiana. Anabasine is the chief alkaloid of Anabasis aphylla, where it occurs from 1—2% in the shoots and is found to ca 1% in Nicotianaglauca. 

Preparation of Thylakoid Vesicles. Broken (class C) chloroplasts from peas (Pisum sativum) and tobacco (Nicotiana tobacum) were prepared according to the method of Avron (29). The chloroplasts were resuspended in a medium containing 0.4-M sucrose and 10-mM tris(hydroxymethyl)aminomethane (Tris pH 7.5), and stored at liquid nitrogen temperature in the same medium supplementated with 30% v/v of ethylene glycol (30). The chloroplasts (6-mg/mL chlorophyl) were heat inactivated for 3 min 51 °C and then diluted by 1 500 with a double-distilled water that was adjusted to pH 7.7 with Tris buffer. Large thylakoid vesicles were formed due to the swelling process under these hypotonic conditions. The size distribution of the thylakoid vesicles was determined as previously described (18). 

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