Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

NHS-palmitate

Dissolve 6.18g of dicyclohexyl carbodiimide (DCC Chapter 3, Section 1.4) in 10ml of ethyl acetate and add it to the NHS/palmitic acid solution. [Pg.886]

The NHS-palmitate may be purified by recrystallization using ethanol. Dissolve the activated fatty acid in a minimum quantity of hot ethanol. Immediately upon dissolving, filter it through a filter funnel containing a fluted glass fiber filter pad, both of which have... [Pg.886]

Analyze the NHS-palmitate for purity using TLC on silica plates. Develop using a solvent mixture of chloroforrmpetroleum diethyl ether (bp 40-60°C) of 8 2. Excess NHS and NHS-palmitate may be detected by staining with 10 percent hydroxylamine in 0.1 N NaOH, followed after 2 minutes by a 5 percent solution of FeCl3 in 1.2 N HC1 (creates red colored spots). [Pg.887]

Add 2 mg of protein to 44pig NHS-palmitate in 20mM sodium phosphate, 0.15M NaCl, pH 7.4, containing 2 percent deoxycholate. [Pg.888]

See other pages where NHS-palmitate is mentioned: [Pg.886]    [Pg.887]    [Pg.888]    [Pg.577]    [Pg.577]    [Pg.577]    [Pg.578]    [Pg.578]    [Pg.557]    [Pg.557]    [Pg.558]    [Pg.558]   
See also in sourсe #XX -- [ Pg.886 , Pg.888 ]

See also in sourсe #XX -- [ Pg.557 , Pg.558 ]

See also in sourсe #XX -- [ Pg.557 , Pg.558 ]



SEARCH



NHS Ester of Palmitic Acid

Palmitate

Palmitates

Palmitic

Palmitic acid NHS ester

© 2024 chempedia.info