Neutrophil adoptive transfer

Here, we describe how to quantitatively and qualitatively describe neutrophil migratory behavior in a defined peripheral tissue site in vivo using multiple complementary methodologies, including immunohistochemistry (IHC), flow cytometry (FCM), enzymatic activity, and MP-IVM in live mice. We also describe the methods of neutrophil isolation from mouse BM, neutrophil labeling with with cell tracker dyes, adoptive neutrophil transfer, and mixed BMC approaches to study the role of chemoattractant receptors in neutrophil recruitment into the joint in a model of inflammatory arthritis. 

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