Neutral chloride transfer reagent

Neutralize the chloride-free prepared sample from above or a 100-mL fresh sample if already chloride free to about pH 7 with dilute NaOH. Transfer to a casserole and evaporate to dryness. Mix the residue with 2.0 mL phenoldi-sulfonic acid reagent, using a glass rod to help dissolve the solids heat on a hot-water bath if necessary to aid dissolution. Dilute with 20 mL distilled water and then add 6 to 7 mL ammonia until maximum color is developed. If a floc-culent hydroxide forms, dissolve it by adding the EDTA reagent dropwise with stirring. (Alternatively, the sample may be filtered.) Transfer the clear solution to a 50-mL volumetric flask and dilute to volmne with distilled water. 

Since 1983, researcher has been trying to develop and evaluate cationic lipids formulation for efficient gene transfer. N-[l-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) [52], [l,2-bis(oleoyloxy)-3-(trimethylammonio)propane] (DOTAP) [53], 3P[N-(N, N -dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) [54], and dioctadecylamido glycylspermine (DOGS) [55] were widely used as a commercial reagent for cationic lipids. A neutral lipid, Dioleoylphosphatidylethanolamine (DOPE)is also often used in conjunction with cationic lipids to aid in endolysosomal escape [56]. 

By Oxidation of Alcohols.—Barium manganate is readily available and stable, and has been recommended for the oxidation of primary and secondary alcohols to aldehydes and ketones respectively. Chromic acid adsorbed on to silica geP and the recyclable poly[vinyl(pyridinium chromate)] effect the same changes, while acid-stable primary and secondary alcohols are oxidized rapidly using potassium dichromate, sulphuric acid, methylene chloride, and a phase-transfer catalyst." Benzeneseleninic anhydride is an alternative reagent for the oxidation of alcohols under essentially neutral conditions. ... 

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