Neuropeptide Analysis by HPLC

This HPLC procedure is fully compatible with biological and radioim- 

The A280 absorbance of p-endorphin has been used to demonstrate a repetitive HPLC yield of greater than 90% at the nanomole level (Fig. 

Equally good yields ( 85%) at the subpicomole level could be demonstrated with radiolabeled I-vasoactive mtestinal /lolypeptide (VIP) (Morris et aL, 19806). The capability for high-yield handling of subpicomole quantities is of major importance in the neuropeptide field where maximal levels of only picograms per milliliter of extract will be encountered. 

Of equal importance is the capability to handle crude samples with little prepurification (thus minimizing handling or degradative losses). In 

Opioid Activity in Met-enkephalin Equivalents (pmol/ml) of Lumbar CSF following Electrical Stimulation 

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