Neuropathy target esterase catalytic activity

FIGURE 57.9. Inhibition and aging of serine esterases by diisopropylphosphorofluoridate (DFP). The active site serine is organophosphorylated in the inhibition step. Aging results in net loss of an isopropyl group to yield the monoisopropylphosphoryl esterase. This mode of inhibition and aging has been established for acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and neuropathy target esterase catalytic domain (NEST) (Kropp and Richardson, 2007). 

Atkins, J., Luthjens, L.H., Horn, M.L., Glynn, P. (2002). Monomers of the catalytic domain of human neuropathy target esterase are active in the presence of phospholipid. Biochem. J. 361 119-23. 

Li, Y., Dinsdale. D., and Glynn P., Protein domains, catalytic activity, and subcellular distribution of neuropathy target esterase in Mammalian cells, J. Biol. Chem., 278(10), 8820-8825, 2003. 

---