Negative staining tissue fixation

TTHIS CHAPTER IS FOR INDIVIDUALS who have not done electron microscopy (EM) before, but it also contains some new information about specimen preparation that can benefit experienced electron microscopists. It covers the materials and methods necessary to do routine thin sections of embedded specimens however, it does not cover specialized EM techniques such as freeze-fracture, rotary shadowing, negative staining, scanning EM, and serial sectioning. Likewise, this chapter focuses mainly on embryos because a description of the fixation methods for all the various types of fly tissues would require an entire book. This chapter is divided into three main sections ... 

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