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Negative polymerase chain reaction controls

Fig. 9.2 Representative examples of the methylation-specific polymerase chain reaction (MSP) analyses for gene promoter regions. Lanes Lf and M indicate the presence of unmethylated and methylated template, respectively. Placental DNA (PDNA) and in vitro methylated DNA (IMD) served as negative and positive controls, respectively. Water (H) was used to detect contamination. Samples 1, 3, 4, and 7 indicate the presence of a methylated promoter DNA with various degrees of methylation, and samples 2, 5, and 6 represent an unmethylated promoter... Fig. 9.2 Representative examples of the methylation-specific polymerase chain reaction (MSP) analyses for gene promoter regions. Lanes Lf and M indicate the presence of unmethylated and methylated template, respectively. Placental DNA (PDNA) and in vitro methylated DNA (IMD) served as negative and positive controls, respectively. Water (H) was used to detect contamination. Samples 1, 3, 4, and 7 indicate the presence of a methylated promoter DNA with various degrees of methylation, and samples 2, 5, and 6 represent an unmethylated promoter...
See other pages where Negative polymerase chain reaction controls is mentioned: [Pg.365]    [Pg.204]    [Pg.263]    [Pg.204]    [Pg.887]    [Pg.515]    [Pg.522]    [Pg.388]    [Pg.320]    [Pg.103]   
See also in sourсe #XX -- [ Pg.663 ]



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