Nature of the Interactions Between Nucleic Acids and Glassy Carbon

Nature of the Interactions Between Nucleic Acids and Glassy Carbon 

An increased adsorption of ssDNA on GC, (and oxidized/reduced GC) was observed. Taking into account the nature of the film formed on the GC(ox) surface, the higher affinity of ssDNA could be explained by the formation of hydrogen bonds. 

An improved adsorption of DNA bases has been observed at a chemically modified electrode based on a Nafion/ruthenium oxide pyrochlore (Pb2Ru2-x FhxOj-y modified GC (CME). Nafion is a polyanionic perfiuorosulfonated ionomer with selective permeability due to accumulation of large hydrophobic cations rather than small hydrophilic ones. The Nafion coating was demonstrated to improve the accumulation of DNA bases, while the ruthenium oxide pyrochlore proved to have electrocatalytic effects towards the oxidation of G and A. The inherent catalytic activity of the CME results from the Nafion-bound oxide surface being hydrated. The catalytically active centers are the hydrated surface-boimd oxy-metal groups which act as binding centers for substrates [50]. 

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