Static nanospray

For static nanoESI-MS connect the static nanospray source (Protana, Odense, Denmark) to the mass spectrometer and use for each sample to be analyzed a new borosilicate spray capillary (ES380 Medium , PROXEON Biosystems AIS, Odense, Denmark see Note 3). 

To couple a gradient HPLC system to the mass spectrometer, replace the needle adapter for static nanospray by a tip adapter and connect the column to Distal Coated SilicaTips with an ID of 10 p,m (New Objective, Woburn, MA). 

FIGURE 1.1 (A) Schematic of a typical static nanospray apparatus that uses a glass needle... 

Traditional static nanospray can be a time-intensive effort and typically requires the expertise of a skilled operator. Automated methods can increase throughput and involve either traditional capillaries or microfluidic chips. 

GAV thanks James P. Murphy forthe static nanospray data and the staff of New... 

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