Nanoparticles lanthanide chelate containing

Silica is an alternative host for lanthanide chelates. Synthesis of nanosized silica particles by well-estabhshed hydrolysis of tetraethylorthosihcate (TEOS) with ammonium hydroxide is a common route for particle preparation. The transparent inert silica material is an excellent matrix for dye-embedded nanolabels as less spectral interferences are expected from the host material. Multiple lanthanide particles have been prepared for bioanalysis. Nanoparticles have been S3Uithesized by hydrolysis of TEOS with ammonium hydroxide in a microemulsion-containing aqueous solution of N,iV,iV, N -[2,6-bis(3 -aminomethyl-l -pyrazolyl)-phenylpyri-dine]tetrakis(acetate)-Tb(in) (BPTA-Tb ) chelate. In the microemulsion, the aqueous phase containing the lanthanide chelate formed nanodroplets acting as nanoreactors for the synthesis of nanoparticles. Since the size of the nanoparticles is dependent on the size of the droplets, it can be controlled, e.g., by the t3q>e of surfactants and by adjusting the concentration of the reactants [42-46],... 

Luminescent lanthanide ions have numerous practical applications in chemistry and technical devices, some of them involving electrical excitation of the lanthanide ions. Photophysical properties of lanthanide ions as well as perhaps the most important application field, photoluminescence of lanthanide chelates, and lanthanide-containing nanoparticles are treated elsewhere in this volume. 

Fig. 2 Size-dependent luminescence intensity of commercially available europiumflll) chelate-dyed nanoparticles (obtained from Seradyn, IN) in 1 g/L Triton X-100 solution. The luminescence intensity of particulate lanthanide reporters is defined as the equivalent number of europium(III) ions in DELFIA enhancement solution resulting in a signal corresponding to that (uiginating from a single particle. The commercial europiumflll) chelate-dyed nanoparticles obtained from Seradyn, Inc. contain europium(III) tris(2-naphthoyl trifluoroacetonate)...

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