Nalidixic acid analysis

Barbate, F. Morrica, P. Seccia, S. Ventriglia, M. High performance liquid chromatographic analysis of quinolone antibacterial agents. Farmaco, 1994, 49, 407-410 [simultaneous cinoxacin, ciprofloxacin, flumequine, nalidixic acid, norfloxacin, ofloxacin, oxolinic acid, pefloxacin, piromidic acid]... 

Over 120 compounds based on the nalidixic acid structure were subjected to QSAR analysis against one Gram-positive and two Gram-negative bacteria. Somewhat inconsistent results were obtained, probably due to two responses being required before significant inhibition of bacterial growth is observed. 

In 2004, Rayner and coworkers reported a dynamic system for stabilizing nucleic acid duplexes by covalently appending small molecules [34]. These experiments started with a system in which 2-amino-2 -deoxyuridine (U-NH ) was site-specifically incorporated into nucleic acid strands via chemical synthesis. In the first example, U-NH was incorporated at the 3 end of the self-complementary U(-NH2)GCGCA DNA. This reactive amine-functionalized uridine was then allowed to undergo imine formation with a series of aldehydes (Ra-Rc), and aldehyde appendages that stabilize the DNA preferentially formed in the dynamic system. Upon equilibration and analysis, it was found that the double-stranded DNA modified with nalidixic aldehyde Rc at both U-NH positions was amplified 34% at the expense of Ra and Rb (Fig. 3.16). The Rc-appended DNA stabilizing modification corresponded to a 33% increase in (melting temperature). Furthermore, imine reduction of the stabilized DNA complex with NaCNBH, resulted in a 57% increase in T. ... 

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