N-succinyl L-leucine 3-hydroxylase

22 BIOCATALYTIC INTRODUCTION OF CHIRAL HYDROXY GROUPS USING OXYGENASES 

TABLE 22.2 Properties of Fe(ll)/aKG-Dependent Dioxygenases (Fe/aKG-Dos) Acting as Stereoselective Aliphatic Amino Acid Hydroxylases  

L-threonine, (2S,3R)-N-succinyl L-p-hydroxyisoleucine, and N-succinyl L-fftreo-p-hydroxyleucine. L-ffereo-p-Hydroxyleucine is a promising target material for the preparation of cyclic depsipeptides that possess useful physiological activities [23,24]. 

Using a combination of N-succinyl L-leucine 3-hydroxylase (SadA) and LasA, N-succinyl L-amino acid desuccinylase (LasA) found in B. ambifaria strain AMMD, N-succinyl L-leucine was successfully converted into L-fftrco-p-hydroxyleucine with 93% molar yield and over 99% diastereomeric excess [25]. Consequently, this new biocatalyhc production system has advantages in optical purity and reaction efficiency for application in the industrial mass produchon of several useful p-hydroxy a-amino acids. 

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