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N-hydroxysulfosuccinimide sulfo-NHS

EDC-mediated coupling This step is crucial for the success of the immobilization procedure. EDC reacts with the carboxyl groups to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS), efficiency of EDC coupling is increased by the formation of an amine-reactive Sulfo-NHS ester intermediate, which has... [Pg.68]

See other pages where N-hydroxysulfosuccinimide sulfo-NHS is mentioned: [Pg.237]    [Pg.213]    [Pg.172]    [Pg.121]    [Pg.237]    [Pg.213]    [Pg.172]    [Pg.121]    [Pg.271]    [Pg.157]    [Pg.172]    [Pg.216]    [Pg.287]    [Pg.766]    [Pg.147]    [Pg.193]    [Pg.259]    [Pg.152]    [Pg.127]    [Pg.173]    [Pg.239]    [Pg.2155]    [Pg.258]   
See also in sourсe #XX -- [ Pg.12 ]



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4 -sulfo

N-Hydroxysulfosuccinimide,

Sulfo-NHS

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