Myoglobin 159 Pancreas

Figure 4.8 Cation-exchange liquid chromatography of basic proteins. Column, Asahipak ES502C eluent, 20 min linear gradient of sodium chloride from 0 to 500 mM in 50 mM sodium phosphate buffer pH 7.0 flow rate, 1 ml min-1 temperature, 30 °C detection, UV 280 nm. Peaks 1, myoglobin from horse skeletal muscle (Mr 17 500, pi 6.8-7.3) 2, ribonuclease from bovine pancreas (Mr 13 700, pi 9.5-9.6) 3, a-chymotrypsinogen A from bovine pancreas (Mr 257 000, pi 9.5) and 4, lysozyme from egg white (Mr 14 300, pi 11.0-11.4). (Reproduced by permission from Asahikasei data)...

Cytochrome c (bovine heart) Myoglobin (horse heart) Chymotrypsinogen (bovine pancreas) /3-Lactoglobulin (goat milk)... 

MATERIALS. Ribonuclease A (RNase A) (bovine pancreas), cytochrome C (Cyt. C) (horse heart), lysozyme (chicken egg white), myoglobin (sperm whale), P-lactoglobulin A... 

The total amount of iron in the body of an adult is about 3-5 g. The highest concentrations are found in the blood (haemoglobin), liver and spleen (ferritin and homosiderin) lower concentrations are in the kidney, heart and skeletal muscle (myoglobin). The concentration of iron in the pancreas and brain is about 2-10 times lower than the content of the Hver or spleen. A list of the organic compounds of iron is given in Table 6.7. 

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