Mutants proline pathway

Oxoglutarate, one of the substrates of hydroxylation, is supplied from glucose in the reaction medium via the EMP pathway and TCA cycle in E. coli and the product succinate is recycled. The mutant strain of E. coli which lacks L-proline-degrading enzyme was obtained and used for the host cells of production of L-hydroxyproline. 

The structural similarities of the 5-carbon amino acids, glutamic acid, ornithine, proline, and hydroxyproline suggested interrelations in their metabolism very early (110). Interconversions of these amino acids were first demonstrated by isotopic studies on the intact rat (111, 112). Later work with mutant strains of microorganisms and with enzyme preparations has revealed the normal biosynthetic pathways from glutamic acid to the... 

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