Specimen preparation muscle

Figure 2 The effects of specimen preparation on structure and element content of biological tissues. (A) Conventionally prepared section of heart muscle. Individual cells, their nuclei, the mitochondria, the Z-lines, and the myofilaments are clearly seen. (B) A heart muscle cell prepared by cryofixation and cryosectioning. Although the cellular structures are the same as in (A), the details are not as distinct. (C) A spectrum derived from material as in (A). The elements that can be Identified are Cu from the grid and Os, Pb, and U from the stain. The only element that can be detected from the specimen itself is S. (D) A spectrum derived from cryoprepared heart tissue. Here the major inorganic elements - Na, Mg, P, S, Cl, and K - are detected.

Preparation of T. spiralis Specimens for Immuno-Localization Studies of Premature and Mature Muscle Larvae and Intact Nurse Cells This procedure was adapted from that used for C. elegans (Miller and Shakes 1995). 

Figure 1 illustrates the preparation steps of the spherical bone samples. Three left femurs were obtained from 29, 30 and 30-month-old female bovines. The muscle and periosteum and bone marrow were removed from the cortical bone using surgical instruments. A ring-shaped cortical bone specimen was obtained from the mid-shaft. We made spherically shaped specimens obtained from anterior part of the cortical bones. The diameter of each spherical specimen was 10 mm. We defined each specimen as specimen 1,2 and 3. 

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