Muscarinic receptor fragments

Maggio, R., Vogel, Z., Wess, J. Reconstitution of functional muscarinic receptors by coexpression of amino-terminal and carboxyl-terminal receptor fragments. FEES Lett., 1993, 319, 195-200. 

Fig. 1. Schematic representation of the wild-type human Mj and rat Mj receptors, the Mj-trunc and Mj-tail fragments, and the mutants Mj-short and MjfAsndCM Ser) muscarinic receptors. The truncated fragment, M2-trunc, contains the amino-terminal domain, the first five hydrophobic transmembrane regions and the initial portion (56 amino acids) of the third cytoplasmic (i3) loop of the wild-type muscarinic Mj receptor. The M3-tail fragment contains the final portion of the i3 loop (105 amino acids), the last two hydrophobic transmembrane regions and the carboxyl-terminal segment of the wild-type M3 muscarinic receptor. The short construct (Mj-short) represents a receptor in which 196 amino acids of the i3 loop have been deleted the remaining loop is 43 amino acids long. The point mutant MjfAsndOd -> Ser) has the asparagine 404 replaced with serine. The amino acid differences in the transmembrane domains VI and VII of the human muscarinic M2 and the rat muscarinic M3 receptors are presented.

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