Multiplex technologies

Coarse Wavelength Division Multiplexing Technologies and Applications, edited by Hans Joerg Thiele... 

It is estimated that by 2015, many in vitro diagnostic products (IVD) analyses for both the lab and point-of-care will be based on miniaturized and multiplexed technologies, for example, chip technologies (Kalorama, 2008 Matsson, 2008). 

Each microsphere is spectrally identified, and quantification is based on the intensity of the reporter signal. In comparison to conventional ELISA methods, this multiplex technology allows simultaneous quantification of up to 100 proteins in a small sample volume, and provides higher reproducibility than multiple ELISA methods. We have used fhe xMap technology to design multiplex assays for simultaneous quantification of several A and tau isoforms. Preliminary data show equal, or better, diagnostic performance than with ELISA (Lewczuk et al., submitted). 

However, the key feature to tap the full potential of the large scale integration approach is the multiplexing technology allowing for the control of N fluid channels with only 2 log2 N control channels. Based on this principle, a microfluidic storage device with 1,000 independent compartments of approximately 250 pL volume and 3,574 microvalves has been demonstrated [122]. 

S. X. Leng, J. E. McElhaney, J. D. Walston, D. Xie, N. S. Fedatko, and G. A. Kuchel, ELISA and Multiplex Technologies for Cytokine Measurement in Inflammation and Aging Research. The Journals of Gerontology. Series A. Biological Sciences and Medieal Seienees 63 p. 879-884 (2008). 

Table 3 Bead-based multiplex technologies and companies...

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