Multiple reaction monitoring standard curves

Kim et al. [48] developed a rapid, sensitive, and selective LC-ESI-MS/ MS method for fhe deferminafion of lornoxicam in human plasma. Lornoxicam and isoxicam (infernal standard) were extracted from human phasma wifh efhyl acefafe at acidic pH and analyzed on a Sunfire Cis column with the mobile phase of methanol-ammonium formate (10 mM, pH 3) (70 30). The analyte was detected using a mass spectrometer, equipped with electrospray ion source. The instrument was set in the multiple-reaction-monitoring mode. The standard curve was linear (r = 9998) over the concentration range of 0.5-500 ng/ml. The coefficient of variation and relative error for the intra- and inter-assay at four QC level were 0.7% fo -4.2% and —4.5% to 5%, respectively. The recoveries of... 

An LC-MS-MS method to monitor lonafarnib (a novel anticancer drug that inhibits farnesyl transferase) in human plasma. Deuterated internal standard is used proteins are precipitated by acetonitrile. Reverse-phase chromatographic separation is performed using acetonitrile/water/formic acid (50 50 0.05, v/v/v) mobile phase. Time of analysis 8 min. A triple quadrupole tandem mass spectrometer in the positive-ion mode with multiple reaction monitoring is used for detection. The cahbration curve has been established in the 2.5-2500 ng/ml concentration range. The validated method was successfully used in phase I trials of the drug. 

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