Multiple Passes Through Spin Columns - Finding Strongest Binders

4 Multiple Passes Through Spin Columns - Finding Strongest Binders [Pg.113]

CPC Spin Column/ESI-MS Ease of Use, Mixture Analysis, High Speed, Reliability, Uncoupling of CPC from ESI-MS and HPLC ESI-MS [Pg.113]

The GPC spin column/ESI-MS screening protocol rapidly analyzes the ability of small organic molecules to bind non-covalently to target protein molecules. [Pg.113]

The site of non-covalent binding of the ligand to the protein is not directly measurable by GPC spin column/ESI-MS. To directly obtain the binding site, X-ray and NMR techniques are used. Site directed mutagenesis and displacement of known binders coupled with GPC spin column/ESI-MS can be used to identify non-covalent binding sites. [Pg.114]

Comparison of CPC Spin Column/HPLC ESI-MS with Tandem Chromatographic Method of CPC/HPLC ESI-MS [Pg.114]

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