Multiple Development in One Direction

Clearly, the number of re-chromatography steps, the development distance, and the total solvent strength and/or selectivity value of the mobile phase can be freely varied, depending on the separation problems (19), as summarized in Table 8.1. 

MD method Abbreviation Development distance Mobile phase composition 

Fractionation of components into polarity groups, and their optimized separation (followed by detection) by subsequent development steps increases the separating capacity of the chromatographic system. 

In the first step, the eompounds to be separated were moved and reeoneentrated. In subsequent steps, both values eharaeterizing the mobile phase were ehanged 

Unfortunately, the fact that, in addition to the 2-D separation, a further increase in separation performance might be obtained by the use of multiple development 

---