Multinudeated cells

Since cell division is a prerequisite for MN expression, identification of mitosis is crucial. Until 1985, the method was hampered by the difficulty to identify the cells that divided in culture. Fenech and Morley introduced in 1985 the cytokinesis block in the methodology. The CBMN assay is based on the addition of cytochalasin-B, an actin inhibitor and therefore an inhibitor of cytokinesis, allowing the discrimination between cells that did not divide (mononudeated cells or MONO) and cells that divided once (binucleated cells or BN) or more (multinudeated cells) during in vitro culture. MN in mononudeated cells can represent the back ound frequency of MN (the frequency of MN that was present before... 

Osteoclasts are multinudeated cells that destroy bone. For a study of osteoporosis, see Rosen (2003). 

Giant cell fibroblastoma is a rare pediatric dermal or subcutaneous slowly growing tumor with a predilection for children under 10 years of age. Histologically the tumor consists of proliferating spindled cells mixed with multinudeated giant cells surrounded by solid fibromyxoid stroma dissected by vascular like spaces. 

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