Multienzyme Catalysis in Microreactors

Microreactors are ideal for directing complex enzymatic synthesis, such as multienzyme catalysis and cascade reactions. There have been a grovdng number of studies [173-178] that represent the implementation of microreactions for multistep enzymatic catalysis and a list is presented in Table 10.5. Microfluidic biocatalytic [Pg.362]

Biotin-avidin D and biotin-streptavidin immobilization inside PDMS Glucose oxidase and horseradish peroxidase (i) 6-D-gluconolactone and hydrogen peroxide (ii) resorufin [173] [Pg.363]

Covalent attachment to PMA-coated glass microchip Invertase, glucose oxidase, and soybean peroxidase poly(p-cresol) [174] [Pg.363]

His-tag affinity on Ni-NTA agarose beads and covalent immobilization on microchannel wall THNS and soybean peroxidase (i) Flaviolin (ii) biflaviolin [175] [Pg.363]

Silica immobilized in microchannel HAB-mutase and soybean peroxidase Chemo-enzymatic synthesis of 2-amino-phenoxazin-3-one with zinc [176] [Pg.363]