Multidimensional Column Liquid Chromatography LC in Proteomics Where Are We Now

Multidimensional Column Liquid Chromatography (LC) in Proteomics - Where Are We Now  

Egidjus Machtejevas, Klaus K. Unger and Reinhard Ditz Abstract 

Edited by M. Hamacher, K. Marcus, K. Stuhler, A. van Hall, B. Warscheid, H. E. Meyer Copyright 2006 Wiley-VCH Verlag GmbH Co. KGaA, Weinheim ISBN 3-527-31226-9 

At the beginning of 2005, it seems useful to recapitulate the state-of-the-art in the proteomics field  

2D polyacrylamide gel electrophoresis (2D PAGE) and MS are weU-established and the most commonly employed techniques in proteomics today. 2D PAGE, however, provides limited information of the total amount of proteins. Low-abimdance proteins and small peptides are not detected [1]. Additional methodologies and techniques in sample preparation, selective enrichment, high resolution separation, and detection need to be developed which would allow even higher resolution than 2D PAGE. Acceptable sensitivity to detect the low-abundance proteins is also still an issue. LC can address some of the above-mentioned 

---