Protease multicatalytic

Hoeeman, L. and Reghsteinee, M. Activation of the multicatalytic protease. The 11 S regulator and 20S ATPase complexes contain distinct 30-kilodalton subunits. J. Biol. Chem. 1994, 269, 16890-16895. 

Driscoll,]., and Goldberg, A. L (1990). The proteasomc (multicatalytic protease) is a component of the 1500-kDa proteolytic complex which degrades ubiquitin-conjugated pto-tein.s. /. Biai Cbetn. 265,4789-4792. 

The 20S proteasome is a multicatalytic protease containing several active centers. These are located in the hole of the cyhnder and are encoded by the j3-subunits. The 20S proteasome acts independently of ATP or any other factor. It is able to degrade unfolded proteins since those can enter the active centers of the proteasome through the opening formed by the a-subunits. This opening is usually covered by domains of the a-subimits and the active center is therefore only accessible after a certain activation of the proteasome. The 20S proteasome is able to cleave proteins on the carboxyl side ofbasic, hydrophobic, and acidic amino acids, described as a trypsin-hke, chymotrypsin-like, and peptidylgluamyl-peptide hydrolase-like proteolytic activity. 

The multicatalytic protease complex consists of a proteolytically active central core (core particle, 20S proteasome) and of a 19S regulatory complex which is attached to the core particle and is responsible for substrate recognition and unfolding. The spatial structure and the en2ymatic mecha-... 

The proteasome field has exploded in the years since the proteasome s discovery in the early 1990s. The proteasome is a highly conserved multicatalytic protease that is responsible... 

The final destination for the majority of polyubiquitinated proteins in the cell is the multicatalytic protease known as the 26S proteasome (Figure 3.3). This high molecular mass enzyme is composed of two primary components the 20S catalytic particle, and the 19S regulatory particle. The 20S catalytic particle... 

Takahashi, M., Tokumoto, T., Ishikawa, K., 1994. DFP-sensitive multicatalytic protease complexes (proteasomes) involved in the control of oocyte maturation in the toad, Bufo japonicus. Mol. Reprod. Dev. 38 (3), 310-317. 

Hoeeman, L., Phatt, G., and Rechsteiner, M. Multiple forms of the 20 S multicatalytic and the 26S ubiquitin/ATP-dependent proteases from rabbit reticulocyte lysate. J. Biol. Chem. 1992, 267, 22362-22368. 

The protease responsible for degradation of ubiquitinated proteins is the proteasome, a 26S multicatalytic endopeptidase complex that hydrolyses proteins... 

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