MS fingerprint

The rapid development and sensitivity of the mass spectrometric methods can be foreseen and in the near future the labeling can be more frequently eliminated. The identification of the cross-linked peptide can be detected first with immunological methods and then the digested and cleaved fragments with specific tandem MS techniques. The different photophores hold discrete MS fingerprints, which allow fast recognition of the modified sites. 

Other MS-fingerprinting techniques that are in commercial development are based on atmospheric pressure ionisation (API), resonance-enhanced multiphoton ionisation (REMPI) TOE and proton-transfer reaction (PTR). They are rapid, sensitive and specific and allow measurements in real time and may play an increasingly important role in the future development of electronic noses and tongues. 

Nicolas, E. C. Scholz,T. H. 1998. Active drug substance impurity profiling part II. LC/MS/MS fingerprinting. J. Pharm. Biomed. Anal., 16, 825-836. 

Nicolas and Scholz [69] described another use of MS-MS in impurity profiling. In the routine monitoring of dmg impurities by means of LC, variation in retention time can lead to uncertainty with respect to the identity of a particular component. The use of the precursor m/z and at least three product ion m/z are used either as MS-MS fingerprint or as diagnostic ions to trace and confirm related substances. 

Oprean, R. Tamas, M. Sandulescu, R. Roman, L. Essential oil analysis. I. Evaluation of essential oil composition using both GC and MS fingerprints. J. Pharm. Biomed. Anal. 1998, 18, 651-657. 

For identification purposes, two basic approaches have been used. In the first, a purified protein is enzymatically digested, the product peptide mixture is analyzed and a mass spectral pattern is obtained. This pattern, called the MS fingerprint , is used to search in internet-available protein or DNA databases.17 Information about protein origin and an estimate of its MW are required in order to improve the chances of a correct match. The search algorithm then theoretically digests all appropriate proteins in the database with the specified enzyme, and matches the... 

The 30/7a-P12 (Jocelyn) condensate is the most mature of all the Pre-Cretaceous petroleums analysed (Fig. 7). Because of its high maturity, many of the biomarkers have been destroyed and so some parameters cannot be quoted. GC-MS fingerprints of the triterpanes (Fig. 10) indicate that only the most thermally resistant molecules Ts, C29TS and C30 diahopane (tt) remain in any abundance. The 30/7a-l Iz and 30/7a-P5z samples from the northernmost part of the field are also highly mature, being more mature than those from the crestal part of the Judy structure, i.e. 30/7a-7, 30/7a-P3 and 30/7a-P9. The 30/7a-P3 fluid appears less mature than that from 30/7a-P9, despite its location within the same crestal fault block, suggesting the possibility of compartmentalization. The decrease in oil maturity from 30/7a-P12 to 30/7a-P5z to 30/ 7a-P9 to 30/7a-P3 is shown in the triterpane... 

Adkins et al. also produced temperature-resolved pyrolysis results for ceU envelope fractions and whole cells of Salmonella typhimurium and for several Salmonella LPS samples. Model compounds (fatty acids, phospholipids, cholines, etc.) for LPS structures were also pyrolyzed using linear-programmed thermal degradation strategies. Common ions were found in the Py-MS fingerprints of cell wall components and the model compounds. Boon et al. °° applied Py-MS to the analysis... 

Wolters, M., Rohde, H., Maier, T Belmar-Campos, C., Franke, G Scherpe, S., Aepfelbacher, M., and Christner, M. (2011) MALDl-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages. Int. J. Med. Microbiol, 301, 64-68. 

Sajewicz, M., Staszek, D., Natic, M., Wojtal, L., Waksmudzka-Hajnos, M., and Kowalska, T. 2011, TLC—MS versus TLC—LC—MS fingerprints of herbal extracts. Part 11. Phenolic acids and flavonoids. J. Liq. Chromatogr. Relat. Technol., 34 864—887. 

Boehme K, Femandez-No IC, Pazos M, Gallardo JM, Barros-Velazquez J, Canas B, Calo-Mata P. Identification and classification of seafood-borne pathogenic and spoilage bacteria 16S rRNA sequencing versus MALDI-TOF MS Fingerprinting. Electrophoresis. 2013 34 877-87. 

Nagy E, Urban E, Becker S, Kostrzewa M, Vbros A, Hunyadkurti J, Nagy I. MALDI-TOF MS fingerprinting facilitates rapid discrimination of phylotypes 1, II and III and Propionibacterium acnes. Anaerobe. 2013 20 20-6. 

Wolters M, Rohde H, Maier T, Belmar-Campos C, Franke G, Scherpe S, Aepfelbacher M, Christner M. MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resis-tant Staphylococcus aureus lineages. Int J Med Microbiol. 2011 301(1 ) 64-8. 

---