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Mowiol mounting medium

Wash each coverslip twice in 0.1% saponin-PBS, once in PBS, and finally once in water to remove salts. Drain all liquid onto a Kimwipe and mount coverslips on microscopy slides by inverting them (cells facing down) on lO-pL drops of Mowiol mounting medium. Allow at least 2 h of polymerization before viewing samples on an epifluorescence microscope. [Pg.142]

Mowiol Mowiol, used as mounting medium, is prepared as described by Harlow and Lane (1988, p. 418) (see also article by Monika Herzog, Annette Draeger, Elisabeth Ehler, and J. Victor Small). Instead of DABCO we use 0.1% p-phenylenediamine as antifade compound. [Pg.145]

Mowiol (Calbiochem, San Diego, CA) mounting medium for immunofluorescence preparations... [Pg.158]

See other pages where Mowiol mounting medium is mentioned: [Pg.136]    [Pg.357]    [Pg.365]    [Pg.136]    [Pg.136]    [Pg.357]    [Pg.365]    [Pg.136]    [Pg.34]    [Pg.106]    [Pg.208]    [Pg.208]   
See also in sourсe #XX -- [ Pg.136 , Pg.142 , Pg.143 ]

See also in sourсe #XX -- [ Pg.359 ]

See also in sourсe #XX -- [ Pg.359 ]

See also in sourсe #XX -- [ Pg.136 , Pg.142 , Pg.143 ]



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Mowiol

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