Morus alba cell cultures

Hano, Y. et al., A novel way of determining the structure of artonin I, an optically active Diels-Alder type adduct, with the aid of an enzyme system of Morus alba cell cultures, J. Chem. Soc. Chem. Commun., 1177, 1992. 

Mulberry tree is a rich source of intermolecular Diels-Alder type adducts coiq>rising two molecules of isoprenylphenols [26]. This article describes the chemistry of the Diels-Alder type adducts from moraceous plants and the biosynthesis of the adducts in Morus alba cell cultures. 

Shlklmate pathway and Isoprenold biosynthesis In Morus alba cell cultures... 

Hano Y, Ayukawa A, Nomura T, Ueda S. Origin of the acetate units composing the hemiterpene moieties of chalcomoracin in Morus alba cell cultures. J Am Chem Soc, 1994 116 4189-4193. 

Morus alba callus and cell suspension cultures specifically produce chalomoradn 5 and kuwanon J 6 from dnnamoylpolyketide intermediates. Administration of [2- C]cinnamic add AT-acetylcysteamine thioester to the M. alba cell cultures revealed the cinnamoyl CoA intermediate to be a significant precursor <99H(50)989>. For further biosynthetic investip-tions see <99H(51)231>. 

Alder reaction, Fig. 36 [85]. Kuwanone J (139) and its related compounds are isolated from Morus alba L. Experiments feeding 4-methoxychalcone into M. alba cell cultures yielded optically active dimethylkuwanone J (140) [86]. This result indicates that the two molecules of prenylchalcone (137) were incorporated into the optically active product (140) by the Diels-Alderase, which a priori catalize dehydrogenation of a chalcone to the diene precursor (138). Accordingly the enzyme would be dehydrogenase, though it has not been identified yet, Fig. 37. 

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