Monomethyl oxyluciferin

INTERACTION OF OXYLUCIFERIN ANALOGS, DIMETHYL OXYLUCIFERIN AND MONOMETHYL OXYLUCIFERIN, WITH FIREFLY LUCIFERASE... 

Firefly luciferase catalyzes oxidation of luciferin with oxygen in the presence of MgATP. Luciferin (substrate) and oxyluciferin (reaction product) are molecules with pronounced fluorescent properties, therefore, fluorescent methods are widely used to study interactions of luciferase with the substrate, the product, and their analogs. Oxyluciferin is extremely unstable in aqueous solutions, however, one may expect that oxyluciferin analogs, dimethyl oxyluciferin (DMOL) and monomethyl oxyluciferin (MMOL), are more stable. Previously we have studied spectral and fluorescence properties of DMOL in aqueous solutions and it was shown that DMOL at alkaline pH undergoes decomposition to form a product with abs = 350 nm and Xem = 500 nm. The goal of this work was to study absorption and fluorescence spectra of MMOL and stability of MMOL and DMOL in aqueous solutions, and in the complexes with the wild-type and mutant (His433Tyr)... 

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See also in sourсe #XX -- [ ]

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