Monoiodized molecules

Monoiodized molecules can be separated from uniodized and multi-iodized molecules via HPLC (Bidard et al. 1989) or isoelectric focusing (Rehm and Betz 1982). The isolation of the simply iodized molecule (from a mixture of simply iodized and uniodized molecules) not only provides for a higher specific activity and a better signal/space ratio but simpbfies the interpretation of the binding results. The amount A of a radioactive compoimd at time t is calculated as follows ... 

If the monoiodized molecule species cannot be isolated, you have a mixture of iodized and uniodized molecules (i.e., you have to determine the specific radioactivity). This is calculated as the amoimt of incorporated radioactivity divided by the amoimt of molecules (iodized + uniodized) in mol. The specific activity is given in Ci/mM. On the basis of the specific activity, you can calculate how many atoms of the radioactive isotope are contained in a molecule (Figure 2.2). Thus, if you have a protein derivatized with iodine and with a specific radioactivity of 2,200 Ci/mM, each protein molecule contains one iodine atom on average. 

An iodination reaction often creates a mixture of different molecules. For example, the iodination of proteins that contain several tyrosine residues yields monoiodized, di-iodized. 

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See also in sourсe #XX -- [ ]

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