Monoclonal antibodies spleen cell preparation

Antibody-producing cells are induced in mice by a series of i.p. injections of POase (20 fig) emulsified with an equal volume of Freund s complete adjuvant (Section 5.3.3.1). The final injection (3 days before removal of spleen) is given i.v. (200 fig POase in saline). The preparation of hybridomas and the selection and propagation of cells producing monoclonal anti-POase antibodies are by the methods given in Section 5.5. 

Figure 13.5 Outline of the production strategy of CEA-SCAN. The antibody-producing hybridoma cell line was originally obtained by standard methods of hybridoma generation. Spleen-derived murine B-lymphocytes were fused with murine myeloma calls. The resulting stable hybridomas were screened for the production of anti-CEA monoclonals. The clone chosen produces an IgG anti-CEA antibody. Note that the finished product outlined above is not radiolabelled. The freeze-dried antibody preparation (which has a shelf life of 2 years at 2-8 °C) is reconstituted immediately prior to its medical use. The reconstituting solution contains 99mTc, and is formulated to facilitate direct conjugation of the radiolabel to the antibody fragment...

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