Molinate organosoluble metabolites

The five major organosoluble metabolites in the water were molinate sulfoxide, 3- and 4-hydroxy molinate, 4-keto molinate, and keto hexamethyleneimine (keto HMI). The amount of each metabolite in tap water remained fairly constant (below 2%) throughout the 14 days. In water previously containing fish, molinate sulfoxide was 2.1% 4 days after treatment, while other metabolites remained below 2% for the first 7 days. By the 14th day, however, molinate sulfoxide totaled 12.8% and 4-hydroxy and 3-hydroxy molinate were 2.2 and 3.7%, respectively. 

In the presence of carp, molinate was rapidly transformed into more polar metabolites. Twenty-four hours after treatment, conversion of molinate to organosoluble metabolites was extensive (Table II). The proportion of molinate sulfoxide increased from 1.2% on day 1 to 10.2% by the 14th day. Molinate, hydroxylated at the 4 position, decreased from 10.8% to 4.6% in 14 days. However, 4-keto and 3-hydroxy molinate together gradually increased over the 14 days from 6.9% to 77.7%. Another metabolite, a keto HMI,... 

Molinate Metabolism in Carp Hepatic Mixed-function Oxidase System. Incubation of molinate with carp liver microsomes produced four major organosoluble metabolites (molinate sulfoxide, 3- and 4-hydroxy molinate, and keto HMI). Parameters affecting... 

In water containing fish, 4-keto molinate constituted 36.8% of all organosoluble metabolites 4 days after molinate addition. However, in the in vitro microsomal mixed-function oxidase system 4-hydroxy molinate was a principal metabolite (14.3%) and 4-keto molinate represented only 0.16% of the total metabolites. Keto-HMI (2.71%) also constituted a substantial proportion of the identified metabolites. 

Figure 10. Chromatographic pattern for organosoluble uC-labeled metabolites from [ring-14C]-molinate-treated water which contained carp for 4 days. F number represents metabolite code.

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