Molecular recognition complexation. thermodynamic parameters

The most promising and most direct, method to diagnose the presence of distinctly digitalis-sensitive isoenzymes in an enzyme preparation would be to determine the rates of formation and dissociation of the complexes between Na /K -ATPase and steroidal inhibitors as a function of their structure. In fact, whenever the thermodynamic equilibrium parameters fail to differentiate, the association and dissociation rates could help towards a distinction, as differential rates are inherently related to distinct specificities. This interconnection, known as the reactivity-selectivity principle from physicochemical studies [251,252], is likewise valid for the dynamics of molecular recognition in biological systems [153,253]. A hypothetical... 

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